Fig. 2: Efficient assembly of three-component nanoparticles from independently purified components for both Fc and IgG.
From: Computational design of non-porous pH-responsive antibody nanoparticles
a, Design models with Fc or IgG (purple), designed nanoparticle-forming tetramers (gray) and pH-dependent plug-forming trimers (yellow). b, Overlay of representative SEC traces of the full assembly formed by mixing designed tetramers, trimers and Fc or IgG (black) with those of the single components in gray (tetramer), yellow (trimeric plug) or purple (Fc or IgG). c, Representative DLS of fractions collected from the O432 assembly peak, showing average hydrodynamic diameters of 34 nm (polydispersity index (PDI), 0.05) and 40 nm (PDI, 0.07) for the O432 assemblies with Fc and full-length IgG, respectively. d, Negatively stained electron micrographs with reference-free 2D class averages along each axis of symmetry in inset; electron microscopy images were collected prior to SEC purification. Scale bars, 100 nm and 10 nm for the micrograph and 2D averages, respectively. The PyMOL Molecular Graphics System version 2.5 (Schrödinger) was used to create a.
