Fig. 3: Cryo-EM analysis of 72-subunit nanoparticles composed of three structural components.
From: Computational design of non-porous pH-responsive antibody nanoparticles
a, Cryo-EM characterization of the O432 assembly with Fc before SEC purification. Computational design models viewed along each axis of symmetry of the octahedral architecture are shown. b, Representative 2D class averages along each axis of symmetry. Scale bars, 10 nm. c, 3D EM density map with 7 Å resolution, reconstructed from the collected dataset. d, Overlay of the design model (gray) and the model refined into the 3D EM density map (pink), showing high agreement. UCSF ChimeraX 1.6 (ref. 54) was used to create a.
