Extended Data Fig. 2: Example experimental screen of O432 three−component nanoparticles.
From: Computational design of non-porous pH-responsive antibody nanoparticles
a, Clarified lysates of 16 designs where the designed trimer co-eluted with the tetramer were supplemented with a purified sfGFP-Fc fusion protein, purified by IMAC, and subject to reducing SDS-PAGE. Trimer protein bands selected for subsequent characterization are marked with pink stars. b, Non-denaturing native PAGE gel electrophoresis of clarified lysates from 16 designs, supplemented with purified sfGFP-Fc fusion protein and purified by IMAC. All designs were compared against the previously designed, two-component antibody nanoparticle (O42.1), O42.1 tetrameric component (O42.1 C4) and sfGFP-Fc (Divine et al.26). Gel samples were derived from the same experiment and electrophoresis was processed in parallel.
