Extended Data Fig. 4: Cryo-EM processing shows newly designed trimeric plug occupying all 3-fold symmetry axes of the nanoparticle octahedral architecture.
From: Computational design of non-porous pH-responsive antibody nanoparticles
a, Representative micrograph of cryo-EM sample. Scale bar, 100 nm. b, Reference-free two-dimensional class averages. Scale bar, 10 nm. c, Ab initio three-dimensional reconstruction without applied octahedral symmetry. d, 3D reconstructions generated following a heterogeneous refinement in the absence of applied octahedral symmetry. All four classes show trimeric plugs occupying all facets of the designed nanoparticle. e, Gold-standard Fourier shell correlation curves for the O432-Fc EM density map with octahedral symmetry applied. f, Close up of plug interface between design model (light gray and yellow) and model built from the 3D reconstruction (dark gray). UCSF ChimeraX 1.6 and the PyMOL Molecular Graphics System, version 2.5 (Schrödinger) was used to create f.
