Extended Data Fig. 10: NONO and PSPC1 regulate CARM1 protein level but not transcription level.
a. Single-cell qPCR results showing neither Nono nor Pspc1 affects the transcription level of Carm1. Two-tailed Student’s t-tests were used for statistical analysis. b. Western blot results showing that Nono or Pspc1 depletion downregulates CARM1 at the protein level. Tubulin is used as a control. Data are presented as mean values ± SEM (n = 3 biological replicates). Two-tailed Student’s t-tests were used for statistical analysis. ns, p > 0.05. α-, anti. kD, kilodalton. c. The ESS level but not the expression level of Carm1 correlates with CARM1 protein level. The protein brightness from dot blotting is calculated by Fiji/ImageJ. Middle, results of dot plotting. Bottom, the relationship between protein brightness and expression level of the ESS isoform Carm1_E56S (Bottom left) or the total expression level of Carm1 (Bottom right) is shown in the dot plot. The Rp values were calculated in Excel. d. The p values for Fig. 5i. One-tailed Student’s t-tests were used for statistical analysis (n = 3 biological replicates).
