Fig. 6: Model of LincGET-guided paraspeckles and PCBP1 on ESS regulation of Carm1 pre-mRNAs. | Nature Structural & Molecular Biology

Fig. 6: Model of LincGET-guided paraspeckles and PCBP1 on ESS regulation of Carm1 pre-mRNAs.

From: Alternative splicing of CARM1 regulated by LincGET-guided paraspeckles biases the first cell fate in mammalian early embryos

Fig. 6

In physiological conditions (first line), LincGET speckles assemble around the Carm1 gene locus and inhibit the access of splicing speckles, leading to less ESS of Carm1 pre-mRNAs and more CARM1 protein. When LincGET loses the HNRNPU-binding domain (second line) or without HNRNPU (third line), LincGET speckles assemble but cannot localize to the Carm1 gene locus, while splicing speckles assemble around the Carm1 gene locus, leading to more ESS of Carm1 pre-mRNAs and less CARM1 protein. When LincGET loses the NONO/PSPC1-binding domain, or without LincGET, Nono or Pspc1 (fourth line), LincGET speckles cannot assemble, while splicing speckles assemble around the Carm1 gene locus, leading to more ESS of Carm1 pre-mRNAs and less CARM1 protein.

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