Extended Data Fig. 2: Cryo-EM data processing of the Rev1-ΔN-Polζ holocomplex. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Cryo-EM data processing of the Rev1-ΔN-Polζ holocomplex.

From: Cryo-EM structure of the Rev1–Polζ holocomplex reveals the mechanism of their cooperativity in translesion DNA synthesis

Extended Data Fig. 2

a, Representative electron micrograph is shown. Scale bar is 200 nm. 8353 micrographs were collected and processed for the cryo-EM reconstruction b, An overview of the workflow for the cryo-EM data processing is shown. Schematic shows particle picking using blob picker and Topaz. Various stages of data processing includes 2-D classification and Ab-initio clean-up using cryoSPARC. The representative 2-D classes are shown and the major 3-D class is used for refinement. The map calculated after non-uniform refinement has a FSC0.143 of 2.85 Å. Number of Particles used at key stages of data processing are shown in blue. c, Cryo-EM density map of the Rev1-ΔN-Polζ holocomplex colored by local resolution is shown. Scale bar is in Å.

Back to article page