Extended Data Fig. 6: Cryo-EM structure determination of the Smc5/6-5mer.
From: Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms
(a) A SEC profile of the Smc5/6-5mer (left) with the peak fraction evaluated by SDS-PAGE (right). The sample purification has been repeated at least three times independently with similar results. (b) A representative micrograph of the Smc5/6-5mer. The microscopic examination has been repeated at least three samples independently with similar results. (c) Representative 2D classification of the Smc5/6-5mer. (d) The workflow of cryo-EM image processing and map reconstruction.
