Extended Data Fig. 4: Cryo-EM structure determination of the Nse1-3-4 subcomplex.
From: Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms
(a) A SEC profile of Nse1-3-4 subcomplex (left) with the peak fraction evaluated by SDS-PAGE (right). The sample purification has been repeated at least three times independently with similar results. (b) A representative micrograph of the Nse1-3-4 subcomplex cryo-EM samples. The microscopic examination has been repeated at least three samples independently with similar results. (c) Representative 2D classification of the Nse1-3-4 subcomplex. (d) The workflow of cryo-EM image processing and map reconstruction of Nse1-3-4 subcomplex. (e) Global Fourier Shell Correlation (FSC) curves (top) and Model vs. map FSC (bottom) of Nse1-3-4 subcomplex. (f) The angular distribution plot (right) and local resolution estimation of Nse1-3-4 cryo-EM map (left).
