Extended Data Fig. 9: Extra densities observed in the SV2A-8783-UCB-2500 and SV2B-PSL cryo-EM maps.
From: Structures of synaptic vesicle protein 2A and 2B bound to anticonvulsants
a. Lipid-like and cholesteryl hemisuccinate densities (light blue) observed close to the intracellular membrane side of SV2A. b. Cholesteryl hemisuccinate density (light blue) in SV2B. c. Glyco-diosgenin and lipid-like densities (light blue) near the luminal side of SV2B. d. Glyco-diosgenin and lipid-like densities (light blue) near the intracellular side of SV2B. e. Unknown density (light pink) observed between several sidechains (sidechain density in light blue) in SV2B, near the intracellular membrane interface. f. Comparison of the central site PSL density (PSL: blue sticks, PSL density: pink) with the unknown density located in the membrane interface site. g. Unknown density (light blue) located between several polar residues (grey sticks) close to the luminal side opening of the central cavity of SV2B, above the central PSL (light blue sticks) binding site. h. Water-like density (light blue, water oxygens as red spheres) in the SV2B near D413. i. Water-like density near G262 in SV2B. j. Water-like density near R462 in SV2B. k. Water-like density near E215 in SV2B. l. Water-like density near N631 in SV2B.
