Extended Data Fig. 3,: Pearson correlation analysis of potential determinants of PspCas13b silencing efficacy.
Pearson correlation analysis between (a) crRNAs silencing efficiency and spacer folding, (b) entire crRNA folding (spacer and direct repeat), (c) target sequence folding, (d) spacer-target hybridization, (e) and spacer-target interaction. Data points in the graph are values of the silencing efficiency (normalized means of independent biological replicates) of individual crRNAs and their predicted folding (MFE) or hybridization/interaction energy. Pearson correlation analysis between spacer silencing efficiency and the content of spacer in (f) A bases, (g) U bases, (h) C bases, (i) G bases, and (j) CG bases. Data points in the graph show the silencing efficiency (normalized means of independent biological replicates) and base content of individual spacer sequences. r (correlation coefficient) and p-value (95% confidence interval) are indicated in each graph. Results are analysed by unpaired two-tailed Student’s t-test. Error bars are SEM. Source data are provided as a Source data file.
