Extended Data Fig. 4: Correlation analysis of PspCas13b potency and spacer nucleotide composition.
(a) The frequency of A, C, G, and U bases in unfiltered crRNA spacer sequences. The frequency (b) and delta frequency (c) of each base in the spacer sequences of filtered potent crRNAs (achieved >90% silencing efficiency). The frequency (d) and delta frequency (e) of each base in the spacer sequences of filtered ineffective crRNAs (achieved <50% silencing efficiency). (f-g) Position Weight Matrices (PWMs) depicting the positional nucleotide probabilities of either the highly potent (f) or the ineffective (g) crRNA spacer sequences. Source data are provided as a Source data file.
