Fig. 5: Validation of prediction accuracy against a human endogenous mRNA.
a, Representative flow cytometry histograms depicting the silencing activity of ten predicted potent crRNAs and ten predicted ineffective crRNAs targeting B2M mRNA compared to an NT crRNA. b, Quantification of the mean fluorescence intensity (MFI) of B2M is shown in the histograms below obtained from n = 3 replicates. The histogram on the right shows the average silencing efficiency of ten potent (white bars) and ten inefficient (gray bars) B2M crRNAs. Data are the normalized means and errors are the s.e.m. Results were analyzed by an unpaired two-tailed Student’s t-test and P values are indicated (95% confidence interval). c, Flow cytometry plots showing the impact of G>C substitutions (red) at the first or first and second nucleotide positions of the spacer (dashed rectangles) of three potent crRNAs targeting B2M (n = 4). Data are the normalized means and errors are the s.e.m. Results were analyzed by an unpaired two-tailed Student’s t-test and P values are indicated (95% confidence interval).
