Extended Data Fig. 2: High variations in the silencing efficiency of 3-nucleotide tiled crRNAs targeting mCherry transcripts. | Nature Structural & Molecular Biology

Extended Data Fig. 2: High variations in the silencing efficiency of 3-nucleotide tiled crRNAs targeting mCherry transcripts.

From: Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing

Extended Data Fig. 2

(a) The schematic illustrates mCherry RNA regions covered by 3-nucleotide increments tiled crRNAs. (b-c) Representative fluorescence microscopy images show the silencing of mCherry transcripts with tiled crRNAs targeting the region surrounding crRNA12 (b) and crRNA 16 (c). Images are processed for quantification using ImageJ. Similar results were obtained in N = 4. Unprocessed representative images are provided in the Source Data file. N is the number of independent biological experiments.

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