Extended Data Fig. 9: Characterization of METTL6 mutants.
a In vitro methylation activity measurements of the different METTL6 mutants. Data is represented as the blank-subtracted mean and standard deviation of independent replicates (n = 3). b Thermal shift assay of METTL6 wild type and mutants, in the presence or absence of SAH. An increase in the melting temperature indicates SAH binding. A representative melting curve of 2 replicates is shown. c Interaction of the different METTL6 mutants with tRNASerUGA measured by fluorescence polarisation. Data is represented as the mean and standard deviation of independent replicates (n = 3), and the sigmoidal fit of the curve. d Complex formation of SerRS mutants, METTL6 mutants and tRNASerUGA qualitatively analysed via analytical size-exclusion chromatography (Column: Superdex 200 3.2/300); representative chromatogramme and gel from one or more replicates. Fractions were analysed for protein and RNA contents using SDS-PAGE and urea PAGE.
