Extended Data Fig. 10: Details of METTL6 - SerRS interaction.
a Close-up view of METTL6 binding of U47D with the corresponding section of the cryo-EM map. b Interaction of METTL6 with different tRNAs measured by fluorescence polarisation. Data is represented as the mean and standard deviation of independent replicates (n = 3), and the sigmoidal fit of the curve. c Close-up view of the interaction between F32 of METTL6 and the N-terminal domain of SerRS with the corresponding section of the cryo-EM map. d Close-up view of the KKMM loop of SerRS with the corresponding section of the cryo-EM map. e Complex formation of SerRS mutants, METTL6 mutants and tRNASerUGA qualitatively analysed via analytical size-exclusion chromatography (Column: Superdex 200 3.2/300); representative chromatogramme and gel from one or more replicates. Fractions were analysed for protein and RNA contents using SDS-PAGE and urea PAGE. f In vitro methylation activity of METTL6 and SerRS mutants on tRNASer and tRNAThr. Data is represented as the blank-subtracted mean and standard deviation of independent replicates (n = 3). g and h Multiple sequence alignment of SerRS sequences in the METTL6 contact loops.
