Fig. 4: The METTL6 active site.
a, Surface slice representation of METTL6 from the cryo-EM structure with the m3C32 and SAH in the active site, which lies in the interface between the methyltransferase core and m3C-RBD. The zoomed-in area shows the Coulomb density around m3C32 and SAH. b, SAH coordination in the interface of the methyltransferase core domain (pale orange) and m3C-RBD (dark orange) in two views. Residues within a 5 Å radius of SAH are represented with side chains as sticks. c, The m3C32-binding site adjacent to the SAH-binding site. Residues within a 5 Å radius of m3C32 are represented with side chains as sticks. d, Methyltransferase activity of single-point mutants in the active site with a qualitative indication of control experiments for complex formation assessed via SEC (Extended Data Fig. 9a), SAH binding by Thermofluor (Extended Data Fig. 9c) and tRNA binding by FP (Extended Data Fig. 9d). Methyltransferase control experiments in Extended Data Fig. 6b. Data are represented as the blank-subtracted mean and s.d. of independent replicates (n = 3). WT, wild type.
