Fig. 1: SpNOX displays the canonical NOX structure and NOX activity.
From: Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase
a, SpNOX is composed of a TM domain (TMD) and a DH domain (DHD) subdivided into an FAD-binding lobe (FBL) and an NADPH-binding lobe (NBL). Nt, N terminus; Ct, C terminus. b, The activity of SpNOX under steady-state conditions was measured using a cytochrome c reduction assay. Apparent KM and kcat values were obtained by fitting the data to the Michaelis–Menten equation. The mean values of three technical replicates are plotted and the s.d. is indicated. c, Schematic view of SpNOX domain boundaries. d, Side view of the cryo-EM map of substrate-free SpNOX. The TMD and DHD are colored blue and coral, respectively. The same coloring is used throughout the article unless otherwise stated. e, Structure of substrate-free SpNOX in cartoon representation. Membrane boundaries are represented as gray lines.
