Extended Data Fig. 7: Cryo-EM of RPc5 °C, I1c5 °C, and I1d5 °C. | Nature Structural & Molecular Biology

Extended Data Fig. 7: Cryo-EM of RPc5 °C, I1c5 °C, and I1d5 °C.

From: Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Extended Data Fig. 7

a.-d. Cryo-EM of RPc5 °C. a. Three views of the combined nominal 3.1 Å resolution cryo-EM map, filtered by local resolution77. The bottom row is colored according to the subunit (αI, αII, light grey; αCTD, limon; β, cyan; β‘, pink; σ70, orange; DNA t-strand, dark grey; DNA nt-strand, grey). The top row shows the same views but colored by local resolution77. The right view is a cross-section through the middle view. b. Directional 3D FSC, determined with 3DFSC90. c. Gold-standard FSC plot95, calculated by comparing two half maps. The dotted line represents the 0.143 FSC cutoff. d. Angular distribution plot, calculated in cryoSPARC. Scale depicts number of particles assigned to a specific angular bin. e.-h. CryoEM of I1c5 °C. e. Three views of the combined nominal 3.4 Å resolution cryo-EM map, filtered by local resolution77. The bottom row is colored according to the subunit (αI, αII, light grey; αCTD, limon; β, cyan; β‘, pink; σ70, orange; DNA t-strand, dark grey; DNA nt-strand, grey). The top row shows the same views but colored by local resolution77. The right view is a cross-section through the middle view. f. Directional 3D FSC, determined with 3DFSC90. g. Gold-standard FSC plot95, calculated by comparing two half maps. The dotted line represents the 0.143 FSC cutoff. h. Angular distribution plot, calculated in cryoSPARC. Scale depicts number of particles assigned to a specific angular bin. i.-l. CryoEM of I1d5 °C. i. Three views of the combined nominal 3.2 Å resolution cryo-EM map, filtered by local resolution77. The bottom row is colored according to the subunit (αI, αII, light grey; αCTD, limon; β, cyan; β‘, pink; σ70, orange; DNA t-strand, dark grey; DNA nt-strand, grey). The top row shows the same views but colored by local resolution77. The right view is a cross-section through the middle view. j. Directional 3D FSC, determined with 3DFSC90. k. Gold-standard FSC plot95, calculated by comparing two half maps. The dotted line represents the 0.143 FSC cutoff. l. Angular distribution plot, calculated in cryoSPARC. Scale depicts number of particles assigned to a specific angular bin.

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