Fig. 2: ATP-mtHsp60V72I structure and client contacts.
a, Top and side view 2D class averages of ATP-bound mtHsp60V72I. Client density (arrow) and the flexible, lower resolution ADs (asterisk) are indicated. Scale bar, 100 Å. b, Sharpened (opaque) and unsharpened (transparent) maps of consensus ATP-bound mtHsp60V72I, colored as in Fig. 1, showing lower-resolution AD density (circled) and the central density corresponding to client (yellow) in the top view (right). c, Cryo-EM processing workflow to capture asymmetric AD conformations. d, mtHsp60ATP focus map, shown as unsharpened mtHsp60 density overlaid with segmented and 8 Å low-pass filtered client density. Note the lack of density for one AD (circled). e, Aligned AD ‘up’ (pink) and ‘down’ (purple) conformations, showing a 25° rigid-body rotation between states. The AD underlying segment (below helices H and I) is indicated (^). f, Unwrapped view of the ADs and client in the mtHsp60ATP focus map from d, showing alternating up and down AD conformations. Note that client extensions (#) are only proximal to ‘down’ protomers (2, 4, 6), and the weak AD density for protomer 7 at the symmetry-mismatched interface. g,h, Putative ‘down’ AD (g) and equatorial domain (h) client contacts. Client is shown as an 8 Å low-pass filtered map and mtHsp60 is shown as models overlaid with transparent unsharpened maps.
