Fig. 3: ATP state mtHsp60–10 structure and client contacts.

a, Sharpened 2.7 Å resolution map of mtHsp60^ATP–mtHsp10, (mtHsp60 colored as in Fig. 1, mtHsp10 in brown). b, Nucleotide-binding pocket of mtHsp60^ATP–mtHsp10, showing density for ATP and the γ-phosphate thereof, and Mg²⁺ and K⁺ ions (gray, from sharpened map). c, Cryo-EM processing workflow to obtain the mtHsp60^ATP–mtHsp10 focus map with resolved client density. d, Slabbed views of the focus map showing the mtHsp10-capped chamber encapsulating client, shown as a segmented, 8 Å low-pass filtered density (yellow). e, Unwrapped view of the mtHsp60^ATP–mtHsp10 focus map from d, showing client contact with multiple ADs (circled). f,g, Enlarged map and model views of AD–client contacts with putative interaction residues labeled (f) and mtHsp60 C-terminal tail-client contacts (maps low-pass filtered to 8 Å) (g).