Extended Data Fig. 6: PspA rod diameter distribution and corresponding cryo-EM structures in the presence of non-hydrolyzable ATP analogs.
From: Structural plasticity of bacterial ESCRT-III protein PspA in higher-order assemblies
Top. PspA rod diameter distribution for A: PspA+ATP (blue), and preformed PspA rods incubated with ATP (brown), C: PspA+ADP (red) and PspA+AMPPCP (magenta), and E: PspA+ATP (blue) and PspA+ATPyS (cyan), based on the relative occurrence of rod segments with a certain diameter. Bottom. Cross-sectional top view z-slices (left column), cross-sectional side view xy-slices (middle column) and FSC curves (threshold 0.143) of cryo-EM structures B: PspA+ATP (preformed), D: PspA+AMPPCP and F: PspA+ATPyS. G: FSC curve of PspA dL10 diameters (threshold 0.143). H: FSC curve of PspA dL10 diameters with ATP (threshold 0.143). Note that when FSC curves drop below 1, systematic peaks of high correlation occur at 1/5.3 Å corresponding to the α-helical pitch and additional low-resolution features of the all α-helical maps of PspA.
