Fig. 2: The structural plasticity of PspA rods increases upon incubation with ADP and ATP. | Nature Structural & Molecular Biology

Fig. 2: The structural plasticity of PspA rods increases upon incubation with ADP and ATP.

From: Structural plasticity of bacterial ESCRT-III protein PspA in higher-order assemblies

Fig. 2

a, Histogram of rod diameters and the relative abundance observed upon addition of ADP (red) during refolding in comparison with PspA apo (gray). In the presence of ADP, the distribution maximum shifts to 250 Å, and diameters up to 400 Å could be observed. The associated cryo-EM structures are shown in the cross-sectional top and side views. b, Histogram of rod diameters upon addition of ATP (blue) during refolding in comparison with PspA apo (gray). In the presence of ATP, the rod population shifts further towards larger diameters in both cases. c, Corresponding three-dimensional cryo-EM atomic models. Dashed-line frames highlight the most frequent structure for apo (215 Å, gray), ADP (250 Å, red) and ATP (270 Å, blue) (α1, red; α2 + 3, violet; α4 blue, α5 cyan).

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