Fig. 5: Changes of EPL vesicle morphologies after SynPspA ± ATP reconstitution.
From: Structural plasticity of bacterial ESCRT-III protein PspA in higher-order assemblies
a, Selected cryo-EM micrograph from 632 recorded movies of EPL control vesicles. b, Selected cryo-EM micrograph from 50 recorded movies of the PspA + EPL sample. Red arrowheads indicate encapsulated vesicles. Blue arrowheads indicate engulfed membranes. c, Violin plot of the bilayer thickness of control EPL SUVs (gray), PspA + EPL SUVs (green) and PspA + EPL + ATP SUVs (orange). The mean bilayer thickness, the number of measured vesicles (n) and P values from a two-sample t-test are indicated on the graph. d, Violin plot of the vesicle perimeter of control EPL SUVs (gray), PspA + EPL SUVs (green) and PspA + EPL + ATP SUVs (orange). The mean vesicle perimeter, the number of measured vesicles (n) and P values from a two-sample t-test are indicated on the graph. e, Box plot of the relative occurrence of double-membrane vesicles in each sample: control EPL SUVs (gray), PspA + EPL SUVs (green), PspA + EPL + ATP SUVs (orange) and PspA (R44K, E126Q, E179Q) + EPL + ATP SUVs (violet). The relative number of double-membrane vesicles, the number of measured vesicles (n) and P values from a two-sample t-test are indicated on the graph (Ctrl versus SynPspA, P = 1.83 × 10−19; Ctrl versus SynPspA + ATP, P = 1.13 × 10−31; Ctrl versus SynPspA R44K/E126Q/E179Q + ATP, P = 1.24 × 10−14; SynPspA versus SynPspA + ATP, P = 0.005; SynPspA + ATP versus SynPspA R44K/E126Q/E179Q + ATP, P = 1.89 × 10−11). IQR, interquartile range.
