Extended Data Fig. 2: Elimination of nucleosome bias in the ATMP-seq assay. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Elimination of nucleosome bias in the ATMP-seq assay.

From: Topoisomerase-modulated genome-wide DNA supercoiling domains colocalize with nuclear compartments and regulate human gene expression

Extended Data Fig. 2

(A) Distribution of ATMP-bound naked gDNA fragments or nucleosome-bound chromatin fragments in an example region at 100-kb resolution. (B) ATMP-seq readout normalized by naked gDNA (ATMP/gDNA) or nucleosome-bound chromatin (ATMP/Chrom), at various spatial resolutions. (C) Correlation between ATMP/gDNA and ATMP/Chrom at various spatial resolutions. (D) ATMP-seq readout (ATMP/gDNA) with different ATMP monoadduct densities (1 monoadduct per 20 kb, 15 kb, or 12 kb).

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