Extended Data Fig. 4: CryoEM data processing for UDP-bound XlHAS1.
From: Structural insights into translocation and tailored synthesis of hyaluronan
a, CryoSPARC workflow for UDP-bound XlHAS1. Scale bar on the micrograph corresponds to 100 nm. b, Local resolution of the final map with orientation distribution plot. c, Map quality for the gating loop and UDP:Mg2+. The map was contoured at σ = 7 r.m.s.d. d, Position of the gating loop in UDP-bound XlHAS1. e, Gating loop in RsBcsA (PDB: 4P00). f, Gating loop in Candida albicans CHS2 (PDB: 7STL). The gating loop is shown in teal. Numbers indicate gating loop residue ranges. Conserved residues are indicated in bold and underlined font.
