Extended Data Fig. 2: CryoEM data processing for apo XlHAS1. | Nature Structural & Molecular Biology

Extended Data Fig. 2: CryoEM data processing for apo XlHAS1.

From: Structural insights into translocation and tailored synthesis of hyaluronan

Extended Data Fig. 2

a, CryoSPARC workflow for apo XlHAS1. Scale bar on the micrograph corresponds to 100 nm. b, Local resolution of the final map with orientation distribution plot. c, Representative regions showing map quality at the indicated contour levels. d, Substrate turnover rates in the presence and absence of the Fab. This experiment was performed in quadruplicates (n = 4) and error bars represent standard deviations from the means. e, HA synthesis in the presence and absence of the Fab and compared to HA standards of defined molecular weight range. f, HAS-Fab interface. Fab binds the GT domain at a position roughly opposite to the active site.

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