Fig. 1: Cryo-EM structure of ApmFz2 ternary complex.
From: Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily
a, Diagram showing target DNA substrate (bottom) annealed to ωRNA (top). Nucleotides not observed in the cryo-EM structure are light gray. The gRNA is pink and the TAM is purple. On the DNA molecule, TS marks the target strand and NTS marks the nontarget strand. b, Top: domain organization of ApmFz2, with domain boundaries indicated by residue numbers. NTD, white; REC, aqua; WED, orange-yellow; RuvC, green; ZnF, pink. Bottom: detailed annotation of the NTD. The NLS was not observed in the cryo-EM map. The RuvC-R (blue-green) and thumb (blue) regions structurally reinforce the RuvC and REC domains, respectively. Gray boxes indicate linker regions not specifically assigned to a domain. c, Cryo-EM reconstruction (top) and atomic model (bottom) of ApmFz2 ternary complex. Domains and nucleic acid molecules are the same colors as in a,b, except for ωRNA, which is shown in white. Insets in d,e are boxed and labeled. d, Close-up view of the thumb domain associated with target DNA in conjunction with the WED and REC domains. A transparent surface is overlaid on the atomic model and the thumb domain is colored according to its electrostatic potential, with blue indicating a highly basic surface. e, View of the active site bound to the precleaved target DNA substrate. The catalytic triad is shown as green sticks and the magnesium ion is shown as a green sphere.
