Fig. 2: ωRNA architecture and structural comparison of existing TnpB structures.
From: Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily
a, A 2D schematic of ωRNA, showing Watson–Crick base pairing (solid lines) and noncanonical interactions. The ωRNA scaffold and guide region span nucleotides −205 to 0 and 1 to 21, respectively. PK, pseudoknot. Disordered regions are in a dashed gray box. b, Comparison of protein and ωRNA structural features across known TnpB ternary complex structures: ApmFz2 (this study), Deinococcus radiodurans TnpB (PDB 8H1J) and Sphenodon punctatus Fanzor1 (PDB 8GKH). Top: protein domain diagram, with domains colored as in Fig. 1a. Middle: RNP complex structure. The ωRNA is colored white. Bottom: the ωRNA structure, with structural features colored as in a, shown in the same view as in the ternary structure (middle row).
