Extended Data Fig. 3: N-Terminal extension in ApmFz2 forms structural contacts with conserved and important functional domains.
From: Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily
a-b. Unique N-terminal extension in ApmFz2 contacting protein functional domains. ApmFz2 domains colored individually (a) or with only N-terminal extension colored in orange (b). Unstructured nuclear-localization signal (NLS) is shown as an orange dotted line. c. Comparison of ApmFz2 and TnpB RuvC domain. RuvC-R domain binds to the canonical RuvC domain. Rotation relative to a is indicated. d. Comparison of ApmFz2 and TnpB linker domain that connects the Rec and Nuc lobes. The N-terminal extension in ApmFz2 forms a short β−sheet with the linker domain. e. Thumb domain forms hydrogen bonds with target strand within and downstream of TAM duplex. Electrostatic interactions are represented as dashed cyan lines.
