Extended Data Fig. 4: Threshold-dependent formation of filamentous GBP1F -GDP·AlF3 protrusions.
From: Structural basis of antimicrobial membrane coat assembly by human GBP1
(a) GBP1 coatomer formation is concentration dependent. A visible coat starts to form at GBP1F concentrations of 7 µM. At a concentration of 15 µM and higher, GBP1-coated tubular protrusions become evident and are the dominant structures at concentrations exceeding 30 µM. (b) Quantification of GBP1-coated protrusions forming on coated SUVs. For each condition, 100 SUVs were randomly selected, classified based on the presence or absence of GBP1-coated protrusions and the fraction of protrusion-forming SUVs was calculated. Mean fractions are displayed; error bars represent a 95% confidence interval from non-parametric bootstrapping. (c) 2D class average of negatively stained GBP1F -GDP·AlF3 tubular protrusion (left panel). The computed power spectrum shows a principal layer line at 0.086 Å-1, corresponding to a periodicity of 11.6 nm along the filament axis (right panel). (d) Formation of micellar assemblies of GBP1F -GDP·AlF3 (white arrows) starts beyond a threshold concentration of around 7 µM.
