Fig. 1: Overview of the in vitro reconstitution experiment.
From: Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes
a, Schematic of the in vitro reconstitution approach. b, XY slices of representative reconstructed tomograms where instances of the inner coat, outer coat and cargo are labeled with blue, red and purple arrowheads, respectively. Bottom left, an example of a vesicle connected to its origin membrane by a neck. Right, two Z slices of the same tomogram. The bottom slice shows both the inner and the outer coat layers of neighboring vesicles. Scale bar, 100 nm. c, Membrane diameters were measured from a control reconstitution reaction where GDP was used and compared with the diameters of coated membranes obtained in the presence of GMP-PNP (n = 1). Box plots centers, boundaries and whiskers represent the median, the 25th and 75th percentiles and the minimum and maximum values within box boundaries + 1.5× the interquartile range, respectively. Statistical significance was determined using a homoscedastic two-tailed t-test (P = 1.7 × 10−8).
