Extended Data Fig. 2: Characterisation of Sed5 cargo.
From: Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes
(A) Alpha-fold2 prediction of the full-length Sed5 structure (PDB ID: AF-Q01590-F1), coloured according to pLDDT value. The C-terminal transmembrane domain is highlighted in green, whilst the two Sec24-binding peptides are highlighted in red. These both fall within very low confidence regions, indicating disorder/flexibility. (B) Acrylamide gel showing purified Sed5 (lane 1), pelleted (lane 2) and floating (lane 3) fractions from a liposome flotation experiment.
