Extended Data Fig. 5: Structural comparisons of the closed CtMrs2 state with relevant conformations of ScMrs2, hMrs2, CorA, ZntB.

a, Alignment of a CtMrs2 monomer (blue) with the AlphaFold model of ScMrs2 monomer (green) (downloaded from AlphaFold Protein Structure Database), including a close-view of the residues lining the Mg²⁺-conducting pathway and certain residues of the NTD. b, The soluble domain of CtMrs2 shows a similar fold as the one previously determined for the NTD of ScMrs2 (cyan, PDB-ID 3RKG). c, Alignments of CtMrs2 (blue) with human hMrs2 (wheat, PDB-ID 8IP3). The left panel shows an alignment of the pentamers, while the right panel displays an alignment of the monomers. d, Alignments of CtMrs2 (blue) with TmCorA (grey, PDB-ID 3JCF). The left panel shows an alignment of the pentamers, while the right panel displays an alignment of the monomers. e, Alignment of CtMrs2 (blue) with ZntB (orange, PDB-ID 5N9Y). The left panel shows an alignment of the pentamers, while the right panel displays an alignment of the monomers. f, Mg²⁺-binding sites (corresponding to site M3 and M4 in CtMrs2) of MjCoA (wheat, PDB-ID 4EV6), and a close-view with residues shown as sticks, and with Mg²⁺ shown as green spheres.