Extended Data Fig. 3: Cryo-EM data processing for the closed CtMrs2 state. | Nature Structural & Molecular Biology

Extended Data Fig. 3: Cryo-EM data processing for the closed CtMrs2 state.

From: Closed and open structures of the eukaryotic magnesium channel Mrs2 reveal the auto-ligand-gating regulation mechanism

Extended Data Fig. 3

a, Representative motion corrected micrographs, more than 2000 micrographs were collected. b, Cryo-EM data processing workflow. c, Selected 2D classes shows the orientation problem with most classes appearing as side-views and with only few tilted views. d, Gold standard Fourier shell correlation curves calculated for the reconstructed map based on a FSC 0.143 cut-off. e, Particle orientation distribution of the final reconstruction with predominant side-view particles. f, Local resolution maps with different views based on a FSC 0.143 cut-off.

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