Fig. 5: Pathogen-induced opening of LoopEFR leads to Pol II readthrough at Xl-k into Ea-IR.
From: Transposon-triggered epigenetic chromatin dynamics modulate EFR-related pathogen response
a, Integrated Genomics Viewer views of Pol IV and Pol V ChIP–seq58 and Pol II nascent elongating transcripts86 in untreated Col-0 wild-type plants. The scale is normalized to total mapped reads. Note that Pol II signal extends beyond the Xl-k termination site, indicated by a vertical orange line. b–d, Expression of the canonical and the long Xl-k transcript isoforms, as measured by RT–qPCR in untreated Col-0 wild-type plants (b), in accessions with Ea-IR (Col-0 and Ba-1) after different time points of infection with Pst DC3000 hrcC (c) and in Col-0, ddc and dcl234 mutants (d). Values of n = 4 (b) and n = 3 (c,d) biologically independent samples are expressed relative to the Col-0 plants. Data are presented as the mean values ± s.d. P values were calculated with a two-tailed unpaired t-test with Welch’s correction.
