Extended Data Fig. 6: Comparison of disome arrangements across different conditions.
From: Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells
a-c, The Cm-treated disome class I is structurally similar to disomes in native untreated M. pneumoniae cells15 (a), while disome classes II (b) and III (c) are more compact. The best resolved disome class in untreated cells (pink, EMD-13287) is shown. All maps are aligned on the leading ribosome (left) for comparison. d-g, Cm-treated disome class III (blue) resembles the in vitro reconstituted collided disomes of B. subtilis27(d, e) and E. coli28(f, g). Aligned on the leading ribosome for comparison. The positioning of mRNA and interface ribosomal proteins largely overlap (e, g). There is a slight rotational difference of the L9 protein. h-l, Comparison of the Cm-treated disome class III to disome structures under different drug treatments15: i, disome average map in M.pneumoniae cells treated with RNA polymerase inhibitor pseudoridimycin (PUM); j, disome average in M. pneumoniae cells treated with the ribosome antibiotic spectinomycin (Spc). k, Overlay of Cm-treated and PUM-treated disome maps shows high similarity. l, Overlay of Cm-treated and Spc-treated disome maps.
