Fig. 3: Cm enriches sequential translation elongation intermediates before peptidyl transfer.
From: Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells
a, Density maps of the seven translation elongation intermediates (six of these are shown) are characterized by differences in tRNA binding (light green, A/T-site; dark green, A-site; blue, P-site; brown, E-site) and the elongation factor EF-Tu (pink). The seventh rotated pretranslocational class ‘A*, P/E’ is not shown here (detailed in Extended Data Fig. 4i). b, Density maps and models of the tRNAs and elongation factor identified in the translation elongation intermediates. A unique class name, for example ‘A, P, E’, is given to each class on the basis of tRNAs and EF-Tu occupancy. The percentage of each class was calculated based on particle numbers. The lower case ‘a’ refers to the flexible aa-tRNA near the A-site, which is only partially resolved (detailed in Extended Data Fig. 4g). c, Cm (orange for density and fitted molecule) was resolved in all six classes, with the corresponding density observed in the canonical binding site in the PTC after fitting the model.
