Fig. 4: Cm induces ribosome collisions within polysomes.
From: Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells
a–c, Three different ribosome pair (disome) arrangements found in M. pneumoniae cells treated with Cm: disome classes I (a), II (b) and III (c), each shown with its percentage among all 70S ribosomes and mRNA exit-to-entry distance (dashed lines). Lower bars, the distributions of translation elongation states for the leading (left) and the following (right) ribosomes of each disome class are calculated based on the ribosome state classification results (Extended Data Fig. 4a). d,e, Map (d) and atomic model (e) of disome class III. The major ribosomal proteins at the interface are S2, S6, S18, S21, S11 and L9 of the leading ribosome (i) and S2, S5 and S4 of the following ribosome (i + 1). Ribosomal protein S1 is not found in M. pneumoniae17. The mRNA (pink) path can be traced threading between ribosomal proteins S21 and S18 of the leading ribosome and S4 and S5 of the following one.
