Extended Data Fig. 5: Spatial and structural analysis of polysomes in Cm-treated M. pneumoniae cells.
From: Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells
a, An example of ribosomes of different functional states classified as described in Extended Data Fig. 4a mapped into the 3D cellular volume. b, The same cell as in panel a colored according to the polysome detection results (light green: polysomes, gray: monosomes). c, Counts of monosomes and polysomes across the 137 cellular tomograms. d, Distribution of polysomes length (number of ribosomes). e, Distribution of translation elongation states in monosomes and polysomes. Bars represent mean percentages and whiskers represent standard deviations across the 137 cells. False discovery rate-adjusted p value (two-sided Wilcoxon rank sum test) is provided. f, The local mask (yellow sphere) used to classify neighboring ribosome pairs (disomes) within the 4,838 spatially annotated polysomes. Aligned on the leading ribosome. g, Comparison of disome classes I and II, after alignment on the leading ribosome (left). The major difference comes from the relative rotation of the following ribosome. h, Comparison of disome classes II and III. The minor difference comes from the displacement and relative rotation of the following ribosome. i, Distribution of the mRNA exiti-to-entryi+1 distances for the three disome classes classified in RELION. The mean (line) and standard deviation (whiskers) of distances for the three disome classes are 4.01 ± 1.27 nm (n = 654 disomes), 2.81 ± 0.91 nm (n = 387), 2.29 ± 0.83 nm (n = 963).
