Extended Data Fig. 8: Structural comparison of VAChT^ACh, VMAT2^5HT and VMAT2^MPP+.

a. Superposition of the overall structures of VAChT^ACh (purple and pink) and VMAT2^5HT (grey). Acetylcholine and 5-HT are represented as cyan and gray spheres respectively. b. Comparison of acetylcholine and 5-HT binding sites between VAChT^ACh and VMAT2^5HT. The substrates and residues involved in the interactions are shown as sticks. c. Superposition of the overall structures of VAChTACh (purple and pink) and VMAT2MPP+ (olive, PDB ID: 8XOA), with acetylcholine and MPP+ represented as cyan and olive spheres, respectively. d. Comparison of acetylcholine and 5-HT binding sites between VAChT^ACh and VMAT2^MPP+. The substrates and residues involved in the interactions are shown as sticks. e. Superposition of the overall structures between VAChT^ACh and VMAT2^5HT with the region of potential protonated sites outlined by a dark dashed circle. f. Comparison of the potential protonated sites between VAChT^ACh and VMAT2^5HT. The putative protonated residues are represented as sticks.