Extended Data Fig. 1: Biochemical characterization and construction strategy of the hVAChT.
From: Binding mechanism and antagonism of the vesicular acetylcholine transporter VAChT
a. Schematic construct and topology diagram of VAChT from N-terminal E27 to the C-terminal T476. Unresolved regions are depicted with dotted lines. TM numbers are labeled, and the plasma membrane is indicated with dashed lines. Inverted repeat motifs TM1-3 (deep blue), TM4-6 (light blue), TM7-9 (red), and TM10-12 (pink) are shown as gray triangles. b. Size-exclusion chromatogram (Superose 6 Increase) profile of the purified VAChT sample. Peak fractions between dashes were used for cryo-EM sample preparation. c. Coomassie-blue-stained SDS-PAGE gel of purified VAChT sample for cryo-EM data collection. VAChT appeared as two bands due to glycosylation. The experiments were independently repeated more than three times, yielding consistent results.
