Extended Data Fig. 1: RTK activation via ERK signaling enhances TK1 expression. | Nature Structural & Molecular Biology

Extended Data Fig. 1: RTK activation via ERK signaling enhances TK1 expression.

From: ERK–USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth

Extended Data Fig. 1

Immunoblotting with the indicated antibodies was performed. (a) The indicated tumor cells were serum-starved for 12 h and then stimulated with or without IGF-1 (100 ng/ml) for the indicated periods of time, quantification of TK1 levels is shown. (b) The GBM cells LN229, U251, U87 were serum-starved for 12 h and then stimulated with or without EGF (100 ng/ml) for the indicated periods of time, quantification of TK1 levels is shown. (c) The indicated tumor cells were serum-starved for 12 h and then stimulated with or without EGF (100 ng/ml) for the indicated periods of time. The mRNA expression levels of the TK1 were measured using qPCR. Data represent the mean ± SD of three biologically independent experiments, analyzed by the one-way ANOVA. (d) Huh7 cells were pre-treated with or without AG1478 (5 μM), U0126 (10 μM), MK2206 (10 μM), SP600125 (25 μM) for 30 min before EGF (100 ng/ml) treatment for 2 h, quantification of TK1 levels is shown.

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