Extended Data Fig. 7: Subtomogram averaging workflow and analyses.
From: Fascin structural plasticity mediates flexible actin bundle construction
a, Subtomogram averaging data processing workflow. Top left: Slice of representative tomogram. Arrowheads indicate fascin cross-bridges. b, Local resolution map of consensus subtomogram average. c, Extreme frames (1 representing 5th percentile, and 10 representing 95th percentile) of interpolation along the first principal component of multi-body refinement. Similar inter-filament rotation is present as was observed in single particle analysis. d, Rigid body docking of the atomic model derived from single particle analysis into the consensus tomogram average, highlighting consistency between these independent analyses. Fascin is displayed in orange and F-actin in blue. e, FSC curves of the consensus (gray) and multi-body refinement reconstructions (yellow, blue). f, Distribution of amplitudes along eigenvector 1 of all subtomograms. g, Distribution of Mahalanobis distances of all subtomograms (n = 129,948). Fit represents gamma distribution (R2 = 0.9951).
