Fig. 7: Accumulation of unfavorable cross-bridges restricts bundle growth.
From: Fascin structural plasticity mediates flexible actin bundle construction
a, Upper, oblique view of fascin cross-bridge Mahalanobis distances calculated from multibody refinement (colored spheres) mapped on to the F-actin bundle. Filaments are displayed in gray. Lower, bundle filament interface scores represented as a weighted graph. b, Dendrogram of graph-based hierarchical clustering of the bundle represented in a; colors represent clusters at linkage distance corresponding to maximum transitivity. c, Plots of graph metrics versus linkage distance. Upper right, quantification of largest, mean and smallest average cluster sizes across bundles. Lower, average cluster transitivity (left) and modularity (right); error bars indicate s.d. Vertical dashed and solid lines indicate linkage distances with maximum transitivity and modularity, respectively, for the bundle shown in a. N = 24 bundles. d, Graphical representation of the clustering at maximum transitivity (upper) and modularity (lower) for the bundle shown in a. e, Cartoon detailing the link between nanoscale conformational flexibility of the fascin cross-bridge and variable micrometer-scale bundle architecture, which limits bundle size.
