Extended Data Fig. 2: Analyses of fascin conformation and bundle element architecture.

a, Superposition of fascin’s four individual β-trefoil domains extracted from the consensus atomic model (Fig. 1f). b, Cryo-EM data processing workflow for reconstructing a hexagonal bundle element. c and d, FSC curves for the bundle element reconstructed with two different box sizes. e and f, Side views of actin planes 1-0-4 (e) and 5-0-2 (f) from the 8.7-Å reconstruction docking model. g-i, Side view of each actin plane of models from 5 bundle element classes aligned on the central filament. j, Distributions of poses of the 6 central-filament associated fascins from the four additional bundle element classes.