Extended Data Fig. 2: Characterization of CEP104 constructs in vitro.
a, Analysis of purified GFP-tagged CEP104 constructs by SDS-PAGE. Asterisks show protein bands. Protein concentrations were determined from a BSA standard. Assays were repeated independently at least two times. b, Fields of view (left, scale bar 2 µm) and kymograph (right, scale bars 2 µm and 60 s) illustrating MT dynamics from GMPCPP-stabilized seed with either HiLyte-488-tubulin or GFP-EB3 and indicated concentrations and colors of CEP104 constructs. c, Co-immunoprecipitation of either CSPP1 (left) or CCDC66 (right) with indicated CEP104 constructs, both CSPP1 and CCDC66 interact with the jelly-roll domain of CEP104. Assays were repeated independently at least two times. d, Analysis of purified GFP-tagged CEP104 ΔJR construct by SDS-PAGE. Asterisk indicates the full-length protein band. Protein concentration was determined from a BSA standard. Assays were repeated independently at least two times.
