Extended Data Fig. 5: Mapping of ARMC9 and CSPP1 interaction.
a, Co-immunoprecipitation experiment of full-length ARMC9 with indicated CSPP1 constructs illustrated in schematic below. Assays were repeated independently at least two times. b, Co-immunoprecipitation experiment of full-length CSPP1 with indicated ARMC9 constructs illustrated in Extended Data Fig. 4a. Assays were repeated independently at least two times. c, Fields of view (left, scale bar 2 µm) and kymographs (right, scale bars 2 µm and 60 s) illustrating MT dynamics from GMPCPP-stabilized seed with GFP-EB3 and indicated concentrations and colors of CSPP1 constructs. Assays were repeated independently three times. d, Co-immunoprecipitation experiment of full-length CSPP1 with full-length TOGARAM1 shows no interaction. Assays were repeated independently at least two times. e, Fields of view (left, scale bar 2 µm) and kymograph (right, scale bars 2 µm and 60 s) illustrating MT dynamics from GMPCPP-stabilized seed with GFP- or mCherry-EB3 and indicated concentrations and colors of CTM proteins. Assays were repeated independently three times.
