Extended Data Fig. 7: Characterization of MT plus ends by cryo-ET. | Nature Structural & Molecular Biology

Extended Data Fig. 7: Characterization of MT plus ends by cryo-ET.

From: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth

Extended Data Fig. 7

a, b, Representative examples of MT plus ends grown in presence of EB3 alone (a) or CTM and EB3 (b). Per MT, the following is shown from left to right; a slice of the denoised tomogram containing the MT plus end, an 8 nm thick transverse cross-section accompanied by rotational averaging analysis to determine MT polarity, and the corresponding 3D model of manual protofilament tracing accompanied by its transverse cross-section. Scale bars 25 nm. Independent experiments were run twice for controls and three time for CTM samples. c, d, Slices (4.3 nm thick) of denoised tomograms showing MTs grown in presence of EB3 alone (c) or the CTM and EB3 (d). Insets show a zoom of the indicated region. Scale bars 100 nm.

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