Fig. 2: A few CEP104 molecules stably block MT plus ends. | Nature Structural & Molecular Biology

Fig. 2: A few CEP104 molecules stably block MT plus ends.

From: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth

Fig. 2

a, Fields of view (top) and histogram plot (bottom) of fluorescence intensities of single GFP molecules, GFP–EB3 dimers and GFP–CEP104 dimers immobilized in separate chambers of the same coverslip. Number of molecules analyzed: GFP, n = 29,981; GFP–EB3, n = 55,378; GFP–CEP104, n = 32,335. Scale bar: 2 µm. b, Representative MT with CEP104-blocked plus end (top) and histogram plot (bottom) of fluorescence intensities of single GFP molecules and GFP–CEP104 intensity at blocked plus end immobilized in separate chambers of the same coverslip. Number of molecules analyzed: GFP, n = 13,925; GFP–CEP104, n = 92. Scale bar: 2 µm. c–e, FRAP analysis of CEP104 (c) and EB3 (d) at blocked MT plus ends or dynamic EB3 at growing plus ends (e). The arrowhead marks the point of photobleaching in representative kymographs. Scale bars: 2 µm and 60 s (c); 2 µm and 10 s (d,e). Plots show averaged curves with exponential fit. Number of FRAP measurements: CEP104, n = 17; stationary EB3, n = 14; dynamic EB3, n = 10. Error bars represent the s.e.m.

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